Lipid-load in circulating blood cells: studying the impact of environmental, dietetic and demographic parameters

Lipid-load in circulating blood cells: studying the impact of environmental, dietetic and demographic parameters

Lipid-load in peripheral blood mononuclear cells (PBMCs) has recently gained attention of the researchers working on nutritional regulation of metabolic health. Previous works have indicated that the metabolic circuitries in the circulating PBMCs are influenced by dietary-intake and macronutrient composition of diet. In Cancer Biology Lab we are studying the factors regulating lipid-load in leukemia cells. To gain better insight into the mechanisms we are also evaluating the factors regulating lipid-load in non-malignant hematopoietic cells. In the initial-phase of this project we analyzed the impact of diet and dietary macronutrients on PBMCs’ lipid-load. The overall analyses revealed that dietary carbohydrates and fats combinatorially induce triglyceride accumulation in PBMCs. On the other hand, dietary fats were shown to induce significant decrease in PBMCs’ cholesterol-load. The effects of various demographic factors –including age, gender and body-weight– on PBMCs’ lipid-load were also examined. Body-weight and age were both shown to affect PBMC’s lipid-load. Our study fails to provide any direct association between extracellular lipid availability and cholesterol-load in both, freshly isolated and cultured PBMCs. The presented work significantly contributes to the current understanding of the impact of food-consumption, dietary macronutrients, extracellular lipid availability and demographic factors on lipid-load in PBMCs

Lipid-load in peripheral blood mononuclear cells. Intra- and inter- individual differences in (a) cholesterol- (left panel) and triglyceride-content (right panel) of PBMCs isolated from healthy volunteers (n=9) in fasting-state. Significance for intra- and inter-individual differences in cholesterol-content (intra-individual, F=0.06989, p=0.9328; inter-individual, F=1.374, p=0.2795) and triglyceride-content (intra-individual, F =2.841, p=0.0922; inter-individual, F=10.50, p=0.0001) was determined by ordinary two-way ANOVA. ­ns; not significant (p > 0.05), *Significant difference (*p=0.0001). Fold-changes in (b)cholesterol- (left panel) and triglyceride-content (right panel) in PBMCs from healthy volunteers (n=14) in response to the cafeteria-diet. Fold-changes in (c) cholesterol- (d) triglycerides-content in PBMCs from healthy volunteers (n=5) in response to the High-Carb, High-Protein or High-Fat diets. Fold-changes in (e) cholesterol- (left panel) and triglyceride-content (right panel) in PBMCs from healthy volunteers (n=10) in response to the High-Fat-High-Carb (HFHC). For figures b-e fold-change in lipid-content was separately calculated for each individual by comparing lipid-load in PBMCs isolated in fasting-state (10-12hr) vs. postprandial state (4hrs after consumption of different diets). Numbers: 1, 2, 3 etc. denote the identification numbers assigned to the study-participants for different experiments.

Related publications from our research group

  1. Ameer F, Munir R, Usman H, Rashid R, Shahjahan M, Hasnain S, Zaidi N: Lipid-load in peripheral blood mononuclear cells: impact of food-consumption, dietary-macronutrients, extracellular lipid availability and demographic factors. Biochimie                                                                         

Related theses from our research group

Level Student’s Name Thesis Title Year
PhD Fatima Ameer Factors affecting lipid and lipidomic profile of malignant and non-malignant hematopoietic cells. Work in progress
Undergraduate Nimrah Farooq Impact of diets with different nutritive values on plasma lipid profiles and lipid-load in peripheral blood mononuclear cells        2016-17
Shajan Qaisirani Lipid homeostasis in peripheral blood mononuclear cells 2015-16